Netherlands voters have declined to support a referendum by European Union in which trade barriers with Ukraine are to be removed.
There was a low turnout of 32.2% but this exceeded the 30% threshold that is needed to make a vote valid. 61.1% of these voters rejected the deal while 38.1% supported it.
PM Mark Rutte said that as much as the vote is not binding, the government may be forced to have second thoughts about the deal.
On the other hand, Ukraine President Petro Poroshenko, said that his country will not relent in its efforts to get closer to the EU.
A foreign ministry official in Kiev told the BBC that the result was disappointing, adding that Dutch Eurosceptics could not take Ukraine hostage to express dissatisfaction with the EU.
The vote was deemed in Netherlands as the position of the public regarding the European Union.
It was as a result of an internet petition that Eurosceptic activists started and ended up attracting over 400,000 signatures.
The result has created more challenges for the Dutch government considering that the Dutch parliament had given a go ahead for the EU association agreement with Ukraine. The EU-Ukraine deal has already been ratified 27 EU member states.
“My view is that if the turnout is more than 30%, with such a victory for the ‘No’ camp, ratification cannot go ahead without discussion,” Mr Rutte said in a televised reaction. It is also an embarrassment for a Dutch government that currently holds the EU presidency.
Geert Wilders, who leads the anti-EU and anti-Islam Freedom Party, said the result was the “beginning of the end for the EU”.